Fig. 4

TNFRSF11A and WNT5A LTR transcripts can be detected in primary cHL tumour tissue and HRS cells. a qPCR measuring TNFRSF11A-LTR and WNT5A-LTR transcript expression performed on RNA extracted from frozen cHL tumour samples (P1–P5) and Reh cells (negative control). Three technical replicates (*p < 0.05 paired Student's t-test). b RT-PCR performed on cDNA from laser capture micro-dissected (LCM) cells from five tumour samples (LCM1–LCM5) comparing HRS cells to bystander cells (NTC), a mixed infiltrate of immune cells. c Gene expression of TNFRSF11A and WNT5A measured by RNA-Seq in the LCM tumour cells