Fig. 1
From: Twins with different personalities: STAT5B—but not STAT5A—has a key role in BCR/ABL-induced leukemia
STAT5B controls transformation and survival of BCR/ABL+ cells. a qPCR for STAT5B in human CML samples. Fold change of STAT5B mRNA levels compared to the mean level of control bone marrow (BM) samples. Control BM was derived from healthy individuals (n = 3); untreated CML patients in chronic phase (CP) (n = 12), acute phase (AP) (n = 4) or blast phase (BP) (n = 3) and relapsed imatinib-resistant patients in CP (n = 5). Error bars represent mean ± SEM. Results were normalized to GAPDH mRNA expression. Levels of significance were calculated using Kruskal–-Wallis test followed by Dunn’s test. b Representative FACS plots (Annexin-V/PI stainings) of BCR/ABLp185+ cells with shRNA-mediated knockdown of Stat5a or Stat5b. Scrambled shRNA was used as control. Right: Percentages of Annexin-V/PI–double positive BCR/ABLp185+ cell lines with shRNA-mediated knockdown of Stat5a or Stat5b (n = 2 cell lines/construct). Error bars represent mean ± SEM. Levels of significance were calculated using Kruskal–Wallis test followed by Dunn’s test. c Co-infection of BM cells with Ab-MuLV (encoding v-Abl) and vectors expressing STAT5A or STAT5B (infection rates are provided in Supplementary Figure 1c) were plated in growth factor-free methylcellulose (n = 2 cell lines/construct). Mean percentages of colonies carrying STAT5A and STAT5B are indicated in pie charts. STAT signal transducers and activators of transcription, BM bone marrow, wt wild type, qPCR quantitative PCR, CML chronic myeloid leukemia, mRNA messenger RNA, PI propidium iodide, shRNA short hairpin RNA
