Fig. 1

Characterization of CD133/PROM1 expression in B-cell ALL and normal HSPCs. a Expression level of PROM1 in the indicated cytogenetic subgroups of B-cell ALL (n = 170 patients at diagnosis) determined by RNA-seq represented in log2(CPM) scale, with CPM = counts per million [13]. b RNA-seq analysis comparing the expression of PROM1 in 11q23/MLLr B-cell ALL (n = 29 patients) with that in distinct fractions of Lin-CD34 + CD38-CD19- non-lymphoid normal HSPCs (HSC hematopoietic stem cells, MPP multipotent progenitors, LMPP lymphoid-primed multipotent progenitors, CMP common myeloid progenitors, GMP granulocyte-monocyte progenitor, MEP megakaryocyte-erythroid progenitors) and in common lymphoid progenitors (CLP) [14]. Data shown as normalized counts. The boxes define the first and third quartiles. The horizontal line within the box represents the median. c Frequency (left) and mean fluorescence intensity (MFI, middle) of CD133+ BM blasts/cells in MLLr (n = 7) and non-MLL B-cell ALLs (n = 5) primary diagnostic/relapse samples or primografts (PDXs), and normal CD34+ HSPCs derived from FL (n = 8), CB (n = 7) and adult PB/BM (n = 7). Representative FACS dot plots for CD133 in normal CD34+ HSPCs (upper right) and BM samples from two independent MLLr B-cell ALL patients (bottom right)