Fig. 2

B12 recognizes both the wild-type and gain-of-function mutant forms of the human IL-7Rα. Cells were incubated with 125 mm (18 μg/mL) B12 and an anti-human IgG alexa 647 secondary antibody and analyzed by flow cytometry: a Ba/F3 cells transduced with either wild-type (wt) or mutant (mut) versions of the mouse or human IL-7Rα. b T-ALL cell lines with different levels of IL-7Rα expression: low/negative (Jurkat, TALL1), intermediate (MOLT4), high (HPB-ALL, IL-7-deprived TAIL7), all of which are IL-7R wild-type; and DND4.1 (high levels of mutant IL-7Rα). c Two primary T-ALL patient samples, either mutant (Patient 1) or wild-type (Patient 2). Red histograms: B12, Blue histograms: secondary antibody only; gray-filled histograms: unstained cells