Fig. 2
From: Efficient treatment of murine acute GvHD by in vitro expanded donor regulatory T cells
Therapeutic transfer of in vitro expanded donor Treg ameliorates established aGvHD and improves survival. a–c CB6F1 recipients were lethally irradiated, transplanted with 2.5 × 106 BALB/c BM cells either alone (BM control; n = 20) or together with 5 × 106 BALB/c splenocytes (GvHD; n = 14) and analyzed 11 days later (time point of Treg application). a Clinical GvHD score (left) and weight change (right) in relation to day 0. b Histopathological score (BM control: n = 6; GvHD: n = 9) and absolute leukocyte numbers in the LP of the colon (BM control: n = 5; GvHD: n = 6) as well as TNF and IFN-γ expression (n = 3 each); mRNA levels determined by qRT-PCR using HPRT as housekeeping gene and normalized to levels in nontransplanted mice. c Treg frequency among donor-derived CD4+ T cells in indicated tissues of GvHD (n = 3–12) and BM control mice (n = 4–20); shaded areas in b and c indicate respective levels in nontransplanted CB6F1 mice. d CB6F1 mice were lethally irradiated and transplanted with BALB/c BM alone (BM control; n = 18) or together with splenocytes (GvHD; n = 23) as stated above. On day 11 after BMT part of the GvHD animals received 5 × 106 in vitro expanded BALB/c Treg (Therapy: n = 48). Survival (left) and clinical GvHD score (right) are shown. Treg application is indicated by the black arrow. e Peak and final clinical scores (maximum score = 2 per parameter) for fur texture (including alopecia), kyphosis, and weight loss of animals surviving for >90 days after Treg therapy (n = 32). Data in a are from 1 representative experiment out of 33, data in b, c are from 1–3 independent experiments and data in d, e are from 7 independent experiments. All summarized data are shown as mean ± s.e.m; *p < 0.05, **p < 0.01, ***p < 0.001
