Fig. 5: Comparison of retinal toxicity induced by NAMPT inhibitors GMX1778 and OT-82. | Leukemia

Fig. 5: Comparison of retinal toxicity induced by NAMPT inhibitors GMX1778 and OT-82.

From: OT-82, a novel anticancer drug candidate that targets the strong dependence of hematological malignancies on NAD biosynthesis

Fig. 5

C57BL/6 mice were treated PO with vehicle (control), GMX1778 (125 mg/kg), or OT-82 at (100 mg/kg) for 5 consecutive days. Mice were weighed on each treatment day and organs were collected 3 h after the last treatment. a Changes in body weight are shown as % change from baseline weight on the first day of treatment prior to dosing (mean ± SE; n = 3 mice/group). b Spleen weight after treatment (mean ± SE; n = 3 mice/group). Asterisks indicate p value <0.01. c Cell density in the outer nuclear layer of the retina. Cells in the outer nuclear layer were counted in histological sections (3–5 slides for the left eye and 2–5 slides for the right eye of each mouse; three mice/group). Triplicate counts of nuclei in 3–5 separate 30 μm × 30 μm squares were made for each slide. Mean ± SE counts were: 69.7 ± 0.4 (n = 147) for vehicle; 52.8 ± 0.4 (n = 102) for GMX1778 and 70.0 ± 0.5 (n = 90) for OT-82. P < 0.0001 for GMX1778 compared with either vehicle or OT-82. P = 0.54 for OT-82 compared with vehicle.

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