Fig. 1: miR-10a regulates several members of the p53/Rb network.

a mRNA expression of p53 pathway modulators and miR-10a targets TFAP2C and RBCC1 in response to miR-10a inhibition (“+ inhibitor”) in the miR-10a-high (/NPM1^c+) OCI-AML3 cell line or miR-10a overexpression (“+ mimic”) in the miR-10a-low (/NPM1^wt) MV4–11 cell line in which p53 was stabilized by Nutlin-3a (“+ Nutlin-3a”) or serum deprivation (“– serum”). b The ability of p53 to upregulate p21 was significantly attenuated in OCI-AML3 cells following upregulation of p21 negative regulator TFAP2C in response to miR-10a inhibition. miR-10a inhibition also significantly increased accumulation of p53 itself (2.4-fold). c A negative correlation was observed between endogenous miR-10a levels and p53 protein levels in NPM1^wt primary AML patient samples. (*p < 0.05, **p < 0.01; ***p < 0.001 by Student’s T test with error bars representing SD (3–6 replicates per experiment); mRNA and protein expression data displayed relative to B-actin, with the scrambled mimic or inhibitor (control)-transfected cells set at 100% for each comparison.).