Fig. 1: MSI2 levels are high in CLL cells and even higher in the proliferative fraction of CLL clones.

A Representative flow cytometry profile of CD19 and CD5 surface expression from a HD and a CLL patient (left, middle panel). MSI2 expression (relative mean fluorescence intensity, MFI-R) in CLL B cells (CD19⁺CD5⁺, green triangles) compared to HD B cells (CD19⁺CD5⁺ or CD19⁺CD5⁻, gray and white circles) (right). B Representative flow cytometry profile of CXCR4/CD5 surface expression on a CLL clone showing the PF (CXCR4^DimCD5^Bright), IF (CXCR4^IntCD5^Int), and RF (CXCR4^BrightCD5^Dim) on the left. Representative flow cytometry histogram of MSI2 expression in PF (red), IF (blue), and RF (green) (middle panel). Relative MSI2 expression in each CXCR4/CD5 fraction in CLL patients from 22 cases (right). C Representative flow cytometry histogram of MSI2 expression in CD19⁺CD5⁺ cells from PB (black line) and LN (orange) (left). The isotype control is shown as a dashed line. MFI-R in CD19⁺CD5⁺ from CLL samples from the different tissues (PB, n = 10 and LN n = 8). D MFI-R in each CXCR4/CD5 fraction. Statistical analysis was performed using One-way ANOVA, Tukey’s multiple comparison test. Shown are individual values. *P < 0.05; ** P < 0.01; ***P < 0.001; ***P < 0.0001.