Table 1 Characteristics of AML samples analyzed by RNAseq.

From: Comprehensive diagnostics of acute myeloid leukemia by whole transcriptome RNA sequencing

Characteristics

No. of AML

Number of patients

100

Age (median, years)

53 (22–77)

Gender

  Male

55

  Female

45

WHO diagnosis

  AML with t(9;11); KMT2A-MLLT3a

4

  AML with inv(16); CBFB-MYH11a

10

  AML with t(8;21); RUNX1-RUNX1T1a

3

  Acute promyelocytic leukemia with t(15;17); PML-RARAa

2

  AML with inv(3); GATA2, MECOMa

2

  AML with t(6;9); DEK-NUP214

1

  AML with mutated NPM1b

35

  Acute monoblastic/monocytic leukemia

11

  AML with myelodysplasia-related changes

13

  AML NOS with maturati

9

  AML NOS with minimal differentiation

1

  AML NOS without maturation

12

  Acute myelomonocytic leukemia

3

  Therapy-related AML

2

AML

  Primary

95

  Relapse

5

Source

  Bone marrow (BM)

78

  Peripheral blood (PB)

22

Blast percentage (median)

75 (13–99)

Karyotypec

  Normal karyotype

45

  Complex karyotype

8

 Abnormal karyotype

37

  No metaphases

2

 FLT3-ITDb

34

  1. aRecurrent fusion transcripts CBFB-MYH11 inv(16)(p13q22), KMT2A-MLLT3 t(9;11)(p21;q23), RUNX1-RUNX1T1 t(8;21)(q22;q22), and PML-RARA t(15;17)(q24;q21) as well as chromosomal translocation GATA2, MECOM inv(3)(q21q26) were detected by fluorescence in situ hybridization (FISH) on 200 interphases per probe set.
  2. bGenotyping for NPM1 and FLT3-ITD mutations was performed by routine diagnostic PCR on genomic DNA followed by electrophoretic fragment size analysis.
  3. cKaryotype was determined by cytogenetics on at least 20 metaphases per case.
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