Fig. 4: H3K79me2/3 regulates enhancer–promoter interactions at PROM1 and TAPT1. | Leukemia

Fig. 4: H3K79me2/3 regulates enhancer–promoter interactions at PROM1 and TAPT1.

From: H3K79me2/3 controls enhancer–promoter interactions and activation of the pan-cancer stem cell marker PROM1/CD133 in MLL-AF4 leukemia cells

Fig. 4

a, b Capture-C from the PROM1 and TAPT1 promoter (gray bars) in control (DMSO; black) and EPZ-5676-treated (DOT1Li; orange) SEM and RS4;11 cells. Differential tracks show the change in Capture-C signal (black: increases; red: decreases). Representative of three biological replicates. ChIP-seq tracks for H3K79me2/3, H3K27ac, and H3K4me1, and ATAC-seq in SEM and RS4;11 cells. c, d Statistical analysis of Capture-C-measured changes in interactions between PROM1 and TAPT1 promoters and enhancers in SEM (c) and RS4;11 (d) cells, from three biological replicates. Each circle represents a KEE (H3K79me2/3-marked enhancer element; purple) or non-KEE (enhancer not marked with H3K79me2/3, gray). Size of circle is inversely proportional to the significance of the change in interaction.

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