Fig. 3: Znhit1 sustains the transcription of HSC quiescence genes to restrict PI3K–Akt signaling for HSC fate determination. | Leukemia

Fig. 3: Znhit1 sustains the transcription of HSC quiescence genes to restrict PI3K–Akt signaling for HSC fate determination.

From: Chromatin remodeler Znhit1 preserves hematopoietic stem cell quiescence by determining the accessibility of distal enhancers

Fig. 3

a Clustered heatmap of differentially expressed genes after Znhit1 deletion. Indicated genes were marked in right. b qRT-PCR was performed to show indicated genes expression in LSKs from control (fl/+) and Znhit1−/− (fl/fl) mice at D5 following pIpC treatment. For qRT-PCR, H3 was used as an internal control (n = 3). c Gene set enrichment analysis (GSEA) of selected gene sets encoding products related to G2/M checkpoint, or E2F targets, presented as normalized enrichment score (NES). Gene expression data come from control (fl/+) and Znhit1−/− (fl/fl) LSKs. d Flow cytometry of the frequency of Akt-phosphorylated (Ser473) in control (fl/+) and Znhit1−/− (fl/fl) LSKs untreated or stimulated with GM-CSF for 5 or 10 min (n = 3). e At D7 following Znhit1 deletion, flow cytometry of BM cells was performed to show the frequency of control (fl/+) and Znhit1−/− (fl/fl) LSKs with/without daily LY294002 treatment for total 7 days after pIpC injection (n = 3). f At D7 following Znhit1 deletion, flow cytometry of BM cells was performed to show the frequency of control (fl/+) and Znhit1−/− (fl/fl) LSKs in G0-G1 phase with/without daily LY294002 treatment for total 7 days after pIpC injection (n = 3). Results were representative of at least three independent experiments. Data were presented as mean ± s.d. *p < 0.05; **p < 0.01; ***p < 0.001.

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