Fig. 2: Dec and 5Aza cause nucleotide imbalances and automatic metabolic compensations for this. | Leukemia

Fig. 2: Dec and 5Aza cause nucleotide imbalances and automatic metabolic compensations for this.

From: Decitabine- and 5-azacytidine resistance emerges from adaptive responses of the pyrimidine metabolism network

Fig. 2

a Experiment schema. Vehicle, natural deoxycytidine (dC) 0.5 μM, natural cytidine (C) 5 μM, Dec 0.5 μM, or 5Aza 5 μM were added once to AML cells at 0 h. b Cell counts. By automated counter. Means ± SD for three independent biological replicates for each cell line. c Dec and 5Aza have opposite effects on dCTP levels. Measured by LCMS/MS 24 h after addition of Dec or 5Aza. Analyses of two or more independent nucleotide extractions from three different AML cells lines. Means ± SD; p values paired t-test, one-sided. d Gene expression 72 h after Dec or 5Aza. Gene expression by QRT-PCR, relative to average expression in vehicle-treated controls. Means ± SD for three independent biological replicates in each of three AML cell lines; p values unpaired t-test versus vehicle, two-sided. e Western blot 72 h after Dec or 5Aza. AML cells THP1, OCI-AML3, and K562. Western blots were reproduced in three independent biological replicates.

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