Fig. 3: DCK is important for maintaining dCTP and UCK2 for maintaining dTTP levels. | Leukemia

Fig. 3: DCK is important for maintaining dCTP and UCK2 for maintaining dTTP levels.

From: Decitabine- and 5-azacytidine resistance emerges from adaptive responses of the pyrimidine metabolism network

Fig. 3

a DCK and UCK2 knockout (KO) were confirmed by Western blot. HAP1 leukemia cells, KO by CRISPR-Cas9. b DCK-KO lowers dCTP and UCK2-KO lowers dTTP. Analysis of independent nucleotide extractions. Means ± SD; p values unpaired t-test, two-sided. c Sensitivity of Wildtype, DCK-KO and UCK2-KO HAP1 leukemia cells to Dec versus 5Aza. Means ± SD of three independent biological replicates.

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