Fig. 4: NPM1mut facilitates nucleolar stress response to dactinomycin. | Leukemia

Fig. 4: NPM1mut facilitates nucleolar stress response to dactinomycin.

From: Dactinomycin induces complete remission associated with nucleolar stress response in relapsed/refractory NPM1-mutated AML

Fig. 4

A Western blot analysis of TP53, NPM1mut and wild-type NPM1 (WT NPM1) in OCI-AML3, IMS-M2, OCI-AML2, and HNT-34 cells, treated with dactinomycin at the indicated concentration (μg/mL) for 6 h. Quantification of TP53 fold changes is reported. See also Fig. S2A. B Cartoon illustrating the design of the experiment performed on OCI-AML2 cells engineered to express doxycycline-inducible NPM1mut. C Western blot analysis of NPM1mut, TP53, P21, and PARP in engineered OCI-AML2 treated with dactinomycin 0.05 μg/mL for 1 h and harvested 3 and 23 h later. Dox doxycycline. ActD dactinomycin.

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