Fig. 1: Active promoters and alternative active promoters in B cell subpopulations and MM patient samples. | Leukemia

Fig. 1: Active promoters and alternative active promoters in B cell subpopulations and MM patient samples.

From: Gene expression derived from alternative promoters improves prognostic stratification in multiple myeloma

Fig. 1

A PCA of the absolute promoter activity of the top 1500 promoters with the highest variance. B Heatmap showing the mean absolute promoter activity for cell-specific active promoters. Data were scaled across cell types for visualization. C Percentage of AP with promoter chromatin state in each cell type. D Genome browser snapshot showing chromatin states and de novo active promoter of YES1 locus in normal B cell subpopulations and MM patients. E YES1 expression levels in B cells subpopulations and MM patient samples. Gene expression is showed in log2 of normalized counts. F Heatmap showing the mean absolute promoter activity for cell-specific alternative promoters (AAP) of genes, which do not change in overall gene expression across cell types. Data were scaled across cell types for visualization. G Percentage of activity of ARID5B alternative promoters (AAP1 and AAP2), explaining the contribution of each promoter to ARID5B total gene expression in each B cell subset. H Total ARID5B expression and its expression derived from each of the two alternative promoters (AAP1 and AAP2) in each B cell subset. Absolute promoter activity and gene expression are in log2 of normalized counts. ARID5B gene expression does not change throughout all B cell differentiation and MM, however, the principal promoter shifts from AAP1 to AAP2 during the differentiation process and the shift to MM. I Genome browser snapshot showing chromatin states (up panel) and expressed alternative transcripts (below panel) from ARID5B locus in normal B cell subpopulations and MM patients. Transcript ID is indicated below the transcript scheme. NB: naïve; GCB: germinal center; CB: centroblast; CC: centrocyte; MEM: memory B cell; TPC: tonsil plasma cell; BMPC: bone marrow plasma cell; MM: plasma cells of MM patient samples; AAP: alternative active promoter. Chromatin States: ActPromt: active promoter; WkPromt: weak promoter; PsPromt: poised promoter; StrEnh1: strong enhancer 1; StrEnh2: strong enhancer 2; WkEnh: weak enhancer; TxnTrans: transcription transition; TxnElg: transcription elongation; WkTxn: weak transcription; Polyc: polycomb; Heterch: heterochromatin; Lowsg: low signal.

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