Fig. 4: VEGF and IGF1 are direct transcriptional targets of JunB in MM cells.

A–C Motif analysis of JunB-enriched sites in MM.1S cells treated with IL6 identified by chromatin immunoprecipitation sequencing (ChIP-seq). A Peak locations across the genome. The number indicates percent of ChIP-seq distribution. B Homer de novo motif results. C Homer known motif enrichment results. D Enriched Gene Ontology (GO) analysis of JunB target genes by Metascape. JunB direct target genes in MM.1S cells treated with IL6 were identified by ChIP-seq and subjected to GO enrichment analysis. E Representative ChIP-seq peaks located on JunB direct target genes VEGF, IGF1, FOXO3, TRAF2, and CLU, visualized by genome browser Integrative Genomics Viewer (IGV). Biological duplicates were performed for the ChIP-seq.