Fig. 2: CXCR4 hyperactivation blocks B-cell differentiation leading to expansion of pre-malignant B cells in cooperation with TCL1.
From: CXCR4 hyperactivation cooperates with TCL1 in CLL development and aggressiveness
a Outline of breeding scheme of CXCR4C1013G and Eµ-TCL1 mice to generate double-transgenic Eµ-TCL1;CXCR4C1013G mice. b Representative contour plots of splenic CD19+CD5+ cells by flow cytometry in 5–6-month-old animals and quantification of splenic and bone marrow CD19+CD5+ cells (Eµ-TCL1, n = 4; Eµ-TCL1;CXCR4C1013G, n = 5). c Spleen weight of 5–6-month-old mice (Eµ-TCL1, n = 4; Eµ-TCL1;CXCR4C1013G, n = 6). d Representative images of H&E and immunohistochemistry of 5–6-month-old Eµ-TCL1 and Eµ-TCL1;CXCR4C1013G mice (scale bars spleen: overview = 1 mm, detailed images: 200 µm, scale bars bone marrow: overview = 200 µm, detailed images = 20 µm). e Quantification and representative contour plots of splenic T1 (IgM+CD23−) and T2 (IgM+CD23+) cells gated on B220+CD93+ B cells by flow cytometry of 3-month-old animals (WT, n = 5; CXCR4C1013G, n = 5). f Quantification and representative contour plots of splenic and bone marrow CD19+B220dimCD5+ B1 cells of 3-month-old animals (WT, n = 5; CXCR4C1013G, n = 5). Statistical analyses were performed with Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate standard deviation (SD).
