Fig. 3: CXCR4 hyperactivation accelerates TCL1-induced lymphomagenesis and dissemination in vivo.
From: CXCR4 hyperactivation cooperates with TCL1 in CLL development and aggressiveness
a Kaplan–Meier survival curves of the indicated cohorts of mice (Eµ-TCL1, n = 18; Eµ-TCL1;CXCR4C1013G, n = 22). Median survival and P value of logrank (Mantel–Cox) test is shown. b Pie charts depicting fractions of animals with overt lymphadenopathy in Eµ-TCL1 (n = 22) and Eµ-TCL1;CXCR4C1013G mice (n = 18). P value of Fisher’s exact test is shown. c Representative images of H&E and immunohistochemistry of Eµ-TCL1 and Eµ-TCL1;CXCR4C1013G with manifest lymphoma (scale bars spleen: overview = 1 mm, detailed images: 200 µm, scale bars bone marrow: overview = 200 µm, detailed images = 20 µm). d Representative images of H&E and immunohistochemistry of a Eµ-TCL1;CXCR4C1013G animal presenting with Ki67 positive Richter-like aggressive lymphoma (scale bars: H&E overview = 200 µm, H&E detailed image = 50 µm, IHC detailed images = 100 µm). e Experimental setup and quantification for ex vivo transwell migration of all splenocytes, CD5+ T cells and CD19+CD5+ cells toward 50 nM CXCL12 isolated from genotypes as indicated (WT, n = 6; CXCR4C1013G, n = 5; Eµ-TCL1, n = 6; Eµ-TCL1;CXCR4C1013G, n = 6). Statistical analyses were performed with one-way ANOVA with Tukey correction for multiple comparisons, *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate standard deviation (SD).
