Fig. 3: YBX1 is a dependency in primary human AML patient samples.
From: YBX1 mediates translation of oncogenic transcripts to control cell competition in AML
A Bar graphs showing the colony counts (top panel) and cell counts (bottom panel) of primary human AML patient samples after genetic inactivation of YBX1 (YBX1-shRNA1/2) or control (SCR-shRNA). After lentiviral transduction cells were plated in methylcellulose (MethoCult™ H4034 Optimum, Stemcell Technologies, Vancouver, Canada) supplemented with 1 µg/ml puromycin at a density of 100.000 cells/4 ml of methylcellulose in a 6-well plate. Colonies were counted and analyzed at 10-14d after plating. B Representative pictures of colonies from 5 primary AML patients after YBX1 inactivation and plating in methylcellulose.
