Fig. 1: MLL-AF9 downregulates Mir139 expression.
From: The tumor suppressor MIR139 is silenced by POLR2M to promote AML oncogenesis
A Flow cytometric analysis of surface marker expression of MLL-AF9 or MLL-AF9-Mir139KO cells stained for myeloid lineage markers CD11b, CD16/32, c-Kit, GR-1 and lymphoid lineage marker CD3. B Heatmap showing the differential gene expression profiles of WT HSPCs (WT), Mir139KO HSPCs (KO), and MLL-AF9 cells (MA9). C The number of colony-forming units (CFUs) per 5000 empty vector control (−) and MLL-AF9 (MA9) transduced WT and Mir139KO HSPCs are shown. Total number of colonies (50–250 cells/colony) and large colonies (>250 cells/colony) are presented. Micrographs depict representative colonies. Scale bar indicates 100 µm. The two-tailed unpaired student’s t test was used for the statistical analysis. D Expression of miR-139-5p and miR-139-3p in MA9 cells and MLL-AF9-Mir139KO relative to snRNA U6 and EV transduced HSPCs (EV) as determined by miRNA qPCR is shown. The two-tailed unpaired student’s t test with Welch’s correction was used for the statistical analysis. The presented data are representative of two experiments. E Expression of Pde2a in MA9 cells and WT HSPCs in fragments per kilobase and per million mapped fragments (FPKM) as determined by RNA-seq is shown. The Wald’s test with Benjamin–Hochberg correction was used for the statistical analysis. All graphs show mean ± SEM.
