Fig. 3: Self-renewal capacities of mutated HSPCs after long-term culture.
From: Modeling clonal hematopoiesis in umbilical cord blood cells by CRISPR/Cas9
A Transfected cells were cultured for up to 9 weeks on irradiated feeder cells. The number of viable cells was determined via trypan blue exclusion and normalized to the number of cells initially plated. B–D Flow cytometry analysis of viable cells after long-term culture. One representative replicate of one biological sample is shown. E Long-term culture initiating cell derived CFU assay. The number of LTC-IC derived CFU was normalized to the number of seeded cells. The y-axis was square root transformed for better visualization. *p < 0.05, **p < 0.01, ***p < 0.001 Wilcoxon rank-sum test corrected for multiple testing.
