Fig. 5: Over expression of miR-15a-5p induces apoptosis in-vitro.
A miR-15a-5p induced apoptosis in KG-1a (green), OCI-AML3 (red), and Ba/F3V600E (blue) cell lines. The cells were stained with Annexin V and propidium iodide (PI) and analyzed using the Muse cell-analyzer. The histograms on the right represent Annexin V-positive cells transfected with a miR-15a-5p mimic or a negative control. Values represent a mean of at least 3 experiments. B Reduced expression of mRNA and C protein levels of the pro-apoptotic Bcl-2 and Bcl-xl proteins. Protein samples were resolved by SDS-PAGE. Bcl-2, Bcl-xl and GAPDH (loading control) antibodies were used to assay protein levels, which were quantified by the Licor imaging system (right graph). Values of protein expression from at least 3 experiments. *p < 0.05, **p < 0.001, ***p < 0.0001.
