Fig. 6: Over expression of miR-15a-5p reduced proliferation and oncogene expression. | Leukemia

Fig. 6: Over expression of miR-15a-5p reduced proliferation and oncogene expression.

From: MicroRNA-15a-5p acts as a tumor suppressor in histiocytosis by mediating CXCL10-ERK-LIN28a-let-7 axis

Fig. 6

KG-1a (green), OCI-AML3 (red), and Ba/F3V600E (blue) cells were transfected with a miR-15a-5p mimic or a negative control for 48 h. A Reduced proliferation of all cell lines overexpressing miR-15a-5p as measured by WST proliferation assay and (B) cell-cycle analysis. C Reduced oncogene expression leading to cell-cycle progression in (C) KG-1a, (D) OCI-AML3, and (E) Ba/F3V600E cells as measured by qRT-PCR. The histograms present the relative expression of each gene from at least 3 experiments as normalized to HPRT1. *p < 0.05.

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