Fig. 5: Synergistic cell death induction by venetoclax and S63845 in BCP-ALL cell lines.
From: Synergistic activity of combined inhibition of anti-apoptotic molecules in B-cell precursor ALL
BCP-ALL cell lines were exposed to increasing concentrations of venetoclax, S63845 or combinations of both inhibitors. A Cell death rates were analyzed by flowcytometry after 48 h by propidium iodide (PI) staining. The heatmaps show cell death rates of dose-response matrix analyses of the cell lines indicated. N = 3 independent experiments in triplicates. B Interaction landscapes of the dose-response matrix analyses are shown. To estimate synergy, δ-scores were calculated using synergyfinder. Synergistic effects are shown in red, additive effects in white and antagonistic effects in green. The shown Bliss synergy score indicates the average synergy score over the dose-response matrix. C Association of the Bliss synergy scores of all cell lines with delta priming response values to BAD + MS1 reflecting combined dependence on BCL-2 and MCL-1. Spearman correlation; r, correlation coefficient; p, significance. D Annexin V and PI staining was assessed upon exposure of cells for 48 h to 10 nM venetoclax and/or 100 nM S63845 to determine apoptotic cells. The fractions of viable cells (Annexin V-/PI-), early apoptotic cells (Annexin V + /PI-), late apoptotic cells (Annexin V + /PI + ) and necrotic cells (Annexin V-/PI + ) are shown. N = 3 independent experiments in triplicates.
