Fig. 4: Silencing IPO11 promotes differentiation of AML cells.
From: IPO11 regulates the nuclear import of BZW1/2 and is necessary for AML cells and stem cells
a Non-specific Esterase (NSE) staining in OCI-AML2 cells 7 days after transduction with shRNA targeting IPO11. The average of the 5 sections for each condition, from three independent experiments is presented. Data are mean ± SD. ***p ≤ 0.001 ****p ≤ 0.0001. b IPO11 protein expression after treatment of NB4 cells with increasing concentration of ATRA for 4 hours. Expression of CD11b by flow cytometry 4 h after treatment with 2 μM of ATRA. c Violin plots for changes in genes associated with AML stemness [19] in TEX cells. d Violin plots for changes in genes associated with AML stemness [19] in primary AML cells (120287). e Pathway analysis of differentially expressed genes IPO11 knockdown in OCI-AML2 cells. Red nodes: pathways significantly enriched in genes upregulated in IPO11 knockdown at FDR 0.05. post-analysis. f ATAC sequencing analysis of global accessible region number in NT cont vs. IPO11 KD in OCI-AML2 cells at FDR 0.05.
