Fig. 7: BZW1/2 KD reduces TYK2 levels.
From: IPO11 regulates the nuclear import of BZW1/2 and is necessary for AML cells and stem cells
a ChIP-SEQ analysis using anti-BZW1 antibody and anti-HA antibody in cell transfected with either HA-tagged BZW1 or HA-tagged BZW2. Significant peaks identified TYK2. b OCI-AML2 cells transduced with sh against IPO11 and analyzed for TYK2 levels by qPCR. c OCI-AML2 cells transduced with sh against BZW1, BZW2, and dual BZW1 and analyzed for TYK2 levels by qPCR. d OCI-AML2 cells were transduced with shRNA targeting TYK2 or control sequences. Cell viability and proliferation were measured over time. TYK2 and actin expression were measured by immunoblotting. e Clonogenic growth of OCI-AML2, cells transduced with shRNA targeting TYK2 or control sequence. Mean ± SD colony counts are shown per ml (750 cells). *p ≤ 0.05, **p ≤ 0.01, by t-test. f OCI-AML2 cells were treated with TYK2 inhibitor for 72 h and assessed for growth and viability by XTT-colorimetric based assay. The presented experiment is representative of three biological repeats. Relative survival is calculated in relation to DMSO control in similar concentrations. g Primary AML cells derived from three different patients were treated with similar concentration of TYK2 inhibitor and assessed for growth and viability. h OCI-AML2 cells were transduced with TYK2 cDNA or empty vector control. Following selection of a stable population, cells were transduced with shRNA targeting IPO11 or control sequences. Equal number of cells were plated and the number of viable cells 5 days after seeding were counted. Levels of TYK2 and IPO11 were measured by immunoblotting.
