Fig. 3: PARP14 is strongly upregulated in IL-4-stimulated STAT6MUT lymphoma cells.
From: PARP14 is a novel target in STAT6 mutant follicular lymphoma
A Volcano plot of differentially expressed genes from whole transcriptome sequencing of OCI-Ly1 cells expressing STAT6WTor STAT6MUT (D419G, D419N, N421K) across all time points (i.e., 2, 4, and 8 h, respectively). Dashed lines indicate log2FC ±1.0, adj. p value 0.0001. B PARP14 expression validated by quantitative PCR (qPCR) in OCI-Ly1 cells. C Immunoblot analysis of PARP14 protein expression in OCI-Ly1 and OCI-Ly8 expressing STAT6WT or STAT6D419G after IL-4 stimulation (10 ng/mL, 24 h). D Immunoprecipitation of 3xFlag-tagged STAT6 and immunoblotting for PARP14 and STAT6 (3xFlag) in OCI-LY8 after IL-4 stimulation (10 ng/mL, 24 h). E Proximity ligation assay (PLA) of STAT6 and PARP14. Representative images of OCI-Ly8 cells expressing STAT6WT or STAT6D419G after IL-4 stimulation (left); TexasRed channel used to detect red amplification signal. Box plots display the percentage of TexasRed positive cells per total cell number for each analyzed microscopic view field (N = 5, right). F Single-cell RNA sequencing analysis. Violin plot showing PARP14 expression in tumor cells from 8 FL patients with wild type STAT6 (STAT6WT, N = 6) vs any STAT6 mutation (MUT, N = 2) vs STAT6 mutation at position D419 (D419MUT, N = 1).
