Fig. 4: Validation experiments in a human ex vivo FL-like co-culture system.
From: PARP14 is a novel target in STAT6 mutant follicular lymphoma
A Schematic of a fully human FL-like model system: human tonsil-derived germinal center (GC) B cells expressing BCL2 and BCL6 as well as STAT6WT or STAT6D419G or EV are co-cultured with follicular dendritic cell (FDC) feeder cells that express CD40L and IL-21 (YK6-CD40lg-IL-21). B Western Blot of FL-like cells, whole cell lysates. C Flow cytometry of GC markers on FL-like cells with indicated STAT6 genotypes. D Western Blot of FL-like cells, cellular and nuclear fraction. E qPCR analysis of FCER2, CCL17, and CCL21, as well as F PARP14 in FL-like cells with indicated STAT6 genotypes with and without IL-4 stimulation.
