Fig. 4: IGF2BP2 enhances NOTCH1 mRNA stability via an m6A-dependent manner.
From: Inhibition of the m6A reader IGF2BP2 as a strategy against T-cell acute lymphoblastic leukemia
A Distribution of m6A peaks and IGF2BP2-iRIP peaks across NOTCH1 mRNA transcript. The overlap region is highlighted in yellow. m6A-seq was repeated once, whereas RIP-sequence was performed twice. B M6A enrichment of NOTCH1 mRNA in Jurkat cells by m6A-RIP-qPCR. Results are presented relative to those obtained with immunoglobulin G (IgG). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), m6A negative control; MYC peak, m6A positive control. C IGF2BP2 expression was measured by western blot in Jurkat cells after RNA pull down assay using single-stranded NOTCH1 RNA with methylated or unmethylated adenosine. Data are mean ± SD values. *P < 0.05; **P < 0.01; ***P < 0.001.
