Fig. 6: AID−/−/Eμ-TCL1 mice exhibited higher levels of CLL infiltration into the peritoneal cavity, lungs, and liver.
From: Loss of AID exacerbates the malignant progression of CLL
A, B Quantification of percentages (A) and numbers (B) of CLL cells in the peritoneal cavities of 6-month-old Eμ-TCL1 (n = 10) and AID−/−/Eμ-TCL1 (n = 7) mice by flow cytometry and cell counting. Peritoneal cavity single cell suspensions were analyzed for CD19+/B220low/CD5+ CLL cells on the gated immune cell populations as determined by positive expression of CD19, CD3, or CD11b. C Representative images of cells collected from the peritoneal cavities of 2 Eμ-TCL1 and 2 AID−/−/Eμ-TCL1 mice at 6 months of age. D Quantification of CLL cells in the lungs of 6-month-old Eμ-TCL1 (n = 10) and AID−/−/Eμ-TCL1 (n = 7) mice by flow cytometry. Lung single cell suspensions were analyzed for CD19+/B220low/CD5+ CLL cells on the gated immune cell populations as determined by positive expression of CD19, CD3, or CD11b. E Quantification of CLL cells in the livers of 6-month-old Eμ-TCL1 (n = 10) and AID−/−/Eμ-TCL1 (n = 7) mice by flow cytometry. Liver single cell suspensions were analyzed for CD19+/B220low/CD5+ CLL cells on the gated immune cell populations as determined by positive expression of CD19, CD3, or CD11b. F Representative images and quantification of CD19+ masses in lung sections from 6-month-old Eμ-TCL1 (n = 8) and AID−/−/Eμ-TCL1 (n = 5) mice by immunohistochemistry. Scale bars: 150 μm. G Representative images and quantification of CD19+ cells in liver sections from 6-month-old Eμ-TCL1 (n = 8) and AID−/−/Eμ-TCL1 (n = 5) mice by immunohistochemistry. Scale bars: 75 μm. H Serum levels of ALT in 6-month-old Eμ-TCL1 (n = 10) and AID−/−/Eμ-TCL1 (n = 10) mice were determined by ELISA.
