Fig. 1: Generation of a germline Zrsr2 allele.
From: Concurrent Zrsr2 mutation and Tet2 loss promote myelodysplastic neoplasm in mice
a Upper panel: Zrsr2 gene consists of eleven exons (boxes). Middle panel: CRISPR/Cas9 construction targeting Zrsr2 exon 10. Lines indicate gRNA sequence (green), PAM motif (blue), and the resulting 17-nucleotides deletion (red). Lower panel: Diagram of WT and truncated ZRSR2 protein with their functional domains: ZF (Zinc Finger), RRM (RNA recognition motif), SR (Serine/Arginine rich domain). Mutant Zrsr2m/m mice expressed a mutated short ZRSR2 protein containing a ZF domain and the RRM domain. The numbers indicate amino acids encoded by WT and mutant protein as predicted in silico. b Relative mRNA abundance of Zrsr2 in bone marrow and spleen determined by RT-qPCR. Data normalized to Hprt. BM: WT (n = 9), Zrsr2m/m (n = 10); SPL: WT (n = 6), Zrsr2m/m (n = 6). c Western blot of FLAG-tagged WT and truncated ZRSR2 protein overexpression in HEK293T cells. Proteins were detected using an anti-FLAG antibody in total cell lysates. Data from two independent experiments.
