Fig. 1: The majority of LSPCs is located in the spleen.

A L-lin⁻ were analyzed in BM, spleen and blood of BL/6 CML mice 18 days after transplantation. One representative experiment out of 5–10 is shown, n = 6 mice per group. B Limiting dilution transplantation of BCR-ABL1-GFP⁺ cells from spleen or BM into lethally irradiated BL/6 mice. C–G Frequencies of LSPC subsets in spleen and BM 18 days after CML induction. One representative experiment out of 5–10 is shown, n = 11 mice per group. H Kaplan–Meier survival curves of secondary BL/6 CML mice. In total, 2 × 10⁴ FACS purified LSCs from spleen or BM were transplanted into sublethally irradiated BL/6 recipient mice. Data are pooled from two independent experiments with n = 13–15 mice per group. I–J Frequencies of L-progenitor cells in spleen and BM 18 days after CML induction. One representative experiment out of 5–10 is shown, n = 5–6 mice per group. K In total, 5 × 10⁴ FACS purified LSCs from BM or spleen were secondarily transplanted into non-irradiated BL/6 mice. LSPCs in BM and spleen of secondary recipients were analyzed 24 days after transplantation. The ratio of the frequency of the individual cell population in spleen versus BM is indicated. Pooled data from 2 independent experiments with n = 5–10 mice per group is shown. Data are displayed as mean ± SEM. Statistics: Unpaired student’s t test (A, C-6, I, J), ELDA (B), log-rank test (H).