Fig. 2: Functional evaluation of T cell reactivity toward neoantigens.
From: Neoantigen reactive T cells correlate with the low mutational burden in hematological malignancies
A Flow cytometry dot plots showing intracellular cytokine staining measuring Interferon-gamma (IFN-γ) and Tumor necrosis factor-alpha (TNFα, left), and CD107a (right) in the CD8 + T cells stimulated with the neoantigen FASGRAGLL, identified to recognize CD8 T cells in MDS patient DD30 (Fig. 1D), or an irrelevant peptide (HLA-C06:01 restricted). Comparative cytokine release profile is shown for cells expanded from the MDS patient DD30 (upper row) and a healthy donor (bottom row). B Similar to (A), intracellular cytokines data measured after stimulation with HLA-C02:02 restricted neoantigen LARAVSAVKNM (identified to recognize CD8 T cells in MDS patient DD31, Fig. 1D). The numbers on the plot indicate the % frequency of CD8+ T cells double or single positive for the analyzed cytokines.
