Table 2 Minimum panels for cell characterization by flow cytometry of Waldenström’s macroglobulinemia at diagnosis.

From: Diagnostics in Waldenström’s macroglobulinemia: a consensus statement of the European Consortium for Waldenström’s Macroglobulinemia

Tube

FITC

PE

PERCP-Cy5.5

PE-Cy7

APC

APC-H7/APC-Cy7

PacB/BV450

PacO/OC515

1

SIgM (5 µl)

CD25 (10 µl)

CD22 (10 µl)

CD19 (5 µl)

CD27 (5 µl)

CD38 (3 µl)

CD20 (5 µl)

CD45 (5 µl)

2

CyIgM (5 µl)

CyL (5 µl)

CD5 (10 µl)

CD19 (5 µl)

CyK (5 µl)

CD38 (3 µl)

CD20 (5 µl)

CD45 (5 µl)

  1. Adapted from Puig et al. [33].
  2. FICT fluorescein isothiocyanate, PE phycoerythrin, PERCP peridinin-chlorophyll-protein complex, Cy5.5 Cyanine 5, Cy7 Cyanine 7, APC allophycocyanin, PacB Pacific Blue, BV450 Brilliant Violet 450, PacO Pacific Orange, OC515 Orange Cytognos 51.
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