Fig. 1: The identification of candidate 6q TS genes.

A Examples of B-ALL patients with deletions of 6q identified by analysis of 250 K SNP array data. Shades of red and blue in the heat maps indicate SNP probes with a normal and reduced copy number respectively. Red boxes contain examples of focal deletions used to define the boundaries of regions 1–4 shown in B. Corresponding chromosome band positions are as indicated on the left and study specific patient IDs for informative focal deletions (Table S3) are shown beneath the heatmaps. B Relative positions of: cytogenetic bands, published common regions of deletion mapped in patients with ALL and recurrent regions of focal deletion based on genomic or SNP array analysis. Green boxes contain symbols for genes within/overlapping the regions of focal deletion and additional genes prioritised for further investigation in functional studies (BACH2 and PRDM1). Red and blue text indicates genomic positions (GRCh38) of deleted regions or candidate tumour suppressor genes, respectively. Region of focal deletion 5, indicated by the orange box containing ARID1B only, did not coincide with any previously published common regions of deletion, and was not investigated further in this study. C Diagrammatic representation of the lentivirus construct SIN-SIEW showing positions of; the spleen focus forming virus promoter (SFFV), internal ribosomal entry site (IRES) and EGFP cDNA. SLIEW, used as a control construct and to facilitate live imaging in-vivo, was constructed by cloning a luciferase cDNA into the indicated BAMH1 site in SIN-SIEW. Using the same BAMH1 site, a ccdB gene flanked by ATTR sites was used to convert SIN-SIEW into a Gateway™ destination vector for recombinational cloning of CCDS (cDNAs). D Overview of the methodology involved in clone tracking assays for the identification of functional tumour suppressor genes.