Fig. 4: Deletions of 6q and functional clone tracking in ETV6::RUNX1-ALL.

A Copy number (CN) profiles of B-ALL patients with abnormalities of 6q and/or Xq, analysed by SNP6.0 array. In all of four patients bearing ETV6::RUNX1 fusion and 6q loss, deletions were terminal and co-occurred with terminal amplifications of Xq in three cases. Terminal amplifications of Xq were seen in the absence of 6q deletion in three ETV6::RUNX1 ALL patients. Terminal and interstitial deletions of 6q or gains of Xq were seen in other B-ALL subtypes but were never co-occurrent in this cohort. Normal CN is indicated by shades of blue or red for chromosomes 6 and X respectively. Dashed vertical lines show the position of PRDM1 and FOXO3 and ideograms of chromosomes 6 and X indicate cytogenetic band positions. Patient IDs are indicated to the left of the heatmaps. Heatmaps were generated using log₂ ratio data in Genotyping Console and rows were scaled individually depending on the clonality of CN abnormalities and sex of patients. B Results of single construct clone tracking assays in 697 and REH cells. Percentage of EGFP positive cells, in cultures transduced with single expression constructs, were determined by flow cytometry at each time point and normalised to the level at day three after transduction. Error bars indicate SEM, * indicates divergence from SIN-SIEW in normalised GFP + ve cell counts with time (p-value < 0.01) in both cell lines.