Fig. 1: MCL-PDLS as a novel 3D model to culture MCL samples ex vivo. | Leukemia

Fig. 1: MCL-PDLS as a novel 3D model to culture MCL samples ex vivo.

From: A novel patient-derived 3D model recapitulates mantle cell lymphoma lymph node signaling, immune profile and in vivo ibrutinib responses

Fig. 1

A Representative scheme showing the workflow for MCL-PDLS generation. Created with BioRender.com. B Brightfield images (magnification ×40) captured in the Cytation 1 of PDLS generated with cytokines (Cyt) and monocytes (Mn) stimuli compared to non-stimulated PDLS control (Ctrl) after 7 days of culture. C 3D reconstruction of a representative PDLS (MCL 1) from an image obtained by SPIM microscopy. D Cell viability in tumor B cells and autologous T cells from PDLS determined by percentage of negative LIVE/DEAD fixable Aqua staining (n = 18) after 7 days of culture. E Cell proliferation in B cells and T cells, calculated as percentage of CFSE low cells, after 7 days of culture (n = 18). F PCA analysis using normalized expression values of six genes related to macrophage polarization obtained by RT-qPCR in macrophages isolated from MCL-PDLS and 2D-differentiated macrophages polarized to M1 or M2 phenotype as references. Undifferentiated monocytes were used as a control. MRC1 and CCL22 are used as M2 markers while CCL5 and CXCL11 are used as M1 markers.

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