Fig. 5: CD40 expression is increased in the lymph node emigrants mediated by TLR9 signaling. | Leukemia

Fig. 5: CD40 expression is increased in the lymph node emigrants mediated by TLR9 signaling.

From: Ibrutinib sensitizes CLL cells to venetoclax by interrupting TLR9-induced CD40 upregulation and protein translation

Fig. 5

A Immunological detection of CD40 in lymph node (LN) single-cell suspension compared to its paired peripheral blood sample (PB) confirmed increased CD40 expression in the LN (N = 3). B Different in vitro stimulations were used to identify which signals are involved in the upregulation of CD40 expression. Stimuli were added to CLL cells for 24 h and expression levels of CD40 were analyzed by flow cytometry. Soluble αIgM + IL-4 (N = 4), αIgM-coated beads + IL-4 (N = 11), CpG (N = 18), TLR4 (N = 10), TLR1/2, TLR3 and TLR7 (N = 7), IL-4 and FAS10 (N = 6), TRAL, CXCL12, TNF, IL-2/10/15/21 (N = 3). Black symbols represent IGHV mutated CLL patients (N = 6), while gray symbols represent IGHV unmutated CLL patients (N = 12). C Combined staining of CXCR4 and CD5 within the CLL population showed increased CD40 expression in the LN emigrant (CD5highCXCR4dim) subsets compared to LN immigrants (CD5dimCXCR4high) upon 24 h CpG (1 µg/ml) stimulation (N = 3). Paired sample t-test was used for statistical analyses.

Back to article page