Fig. 4: CAR-T cell infiltration and transcriptional analysis of solitary extramedullary diseases (sEMD).
From: Bispecific CS1-BCMA CAR-T cells are clinically active in relapsed or refractory multiple myeloma
A Schematic diagram of the EMDs of patient 7 in month 1 after infusion. B T and CAR-T cell analysis of peripheral blood (PB), EMD_1 and EMD_2 of patient 7 by flow cytometry. Cells were gated by forward and side scatter, then singlets (P1), then lymphocytes and/or beads for absolute counting, and then CD3 + T and CAR + CD3 + T cells. C Gene set variation analysis of PB, EMD_1 and EMD_2 of patient 7 in month 1. D Volcano map of differential genes of EMD compared to PB, with marked 10 differentially upregulated genes and 10 differentially downregulated genes. E Volcano map of differential genes of EMD_2 compared to EMD_1, with marked 10 differentially upregulated genes and 10 differentially downregulated genes.
