Fig. 3: DELE1 down-expression modulates OMA1–DELE1–HRI pathway. | Leukemia

Fig. 3: DELE1 down-expression modulates OMA1–DELE1–HRI pathway.

From: DELE1 haploinsufficiency causes resistance to mitochondrial stress-induced apoptosis in monosomy 5/del(5q) AML

Fig. 3

A Schematic representation of the OMA1-DELE1-HRI pathway recently defined by Fessler et al. [10] and Guo et al. [11]. S and L suffixes indicate short and long forms of OPA1 and DELE1. IMM: inner mitochondrial membrane, OMM: outer mitochondrial membrane. B Radar plot summary representation of the significance of sensitivity reduction to oligomycin, cytarabine and thapsigargin (-log10(P-values), Mann-Whitney tests on IC50 values) for OCI-AML-1, OCI-AML-5 and K562 cells (see legend) expressing shRNA vectors targeting DELE1 (shDELE1#1 and shDELE1#2) or the luciferase as control (shLuc). C Oligomycin IC50 values (log10 of concentrations in nM of the compound that inhibited cell growth by 50%) for OCI-AML-1, OCI-AML-5 and K562 cells expressing shDELE1 (shDELE1#1 and shDELE1#2) or shLuc. P-values resulting from Mann-Whitney tests comparing shDELE1#1 and/or shDELE1#2 vs. shLuc conditions are directly indicated on the figure. D Western blot analysis of total proteins extracted from OCI-AML1 (left panel) and HL60 cells (right panel) expressing shDELE1#1 or shLuc (control) and exposed to DMSO or CCCP (20 µM) for 4 and 24 h (hrs). Representative blot showing OPA1, OMA1, DDIT3, eIF2a, (P)eIF2a and TUBULIN as loading control.

Back to article page