Fig. 2: Impact of IκBε loss on the proliferation, survival and NF-κB pathway activation of Eμ-TCL1 CLL cells following CpG stimulation. | Leukemia

Fig. 2: Impact of IκBε loss on the proliferation, survival and NF-κB pathway activation of Eμ-TCL1 CLL cells following CpG stimulation.

From: NFKBIE mutations are selected by the tumor microenvironment and contribute to immune escape in chronic lymphocytic leukemia

Fig. 2

A Analysis of cell proliferation by BrdU incorporation and cell viability by Annexin V/PI staining performed after 24 h culture with 1 μM CpG-ODN 1668. Cells were cultured for 6 h with BrdU prior to harvesting for flow cytometry analysis. Statistical analysis was done using One Way ANOVA with Tukey test for multiple comparisons. B, C Analysis of nuclear translocation of NF-κB transcription factors following CpG-DNA stimulation of NFKBIE-ko and NFKBIE-wt TCL1-355-TKO cells. Nuclear and cytoplasmic proteins were extracted before or after 1 h, 3 h and 5 h CpG-stimulation. One representative experiment is shown in B and a summary of 5 experiments for p65 and 6 for p52, c-Rel and p50 is shown in C. Statistical analysis was done with the paired t test.

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