Fig. 5: Transcriptome analysis of NFKBIE-wild type and NFKBIE-mutated tumors.

A Principal Component Analysis of the transcript expression counts (log2 vst-method normalized counts) for the 22 samples showing the first versus the second PC. PC1 shows 47% variance and PC2 18%. Each dot represents a NFKBIE-wt sample, each triangle is a NFKBIE-ko sample. Right panel shows Venn diagram of DETs. Numbers in each circle represent the number of DETs between the different comparisons while the ones overlapping are for mutual DETs. Red numbers represent upregulated DETs while blue numbers represent downregulated DETs. B Top ten Gene Ontology terms based on the Gene Ratio (x-axis), which is the ratio between the transcripts of the set found to be enriched for that specific term and the total transcripts in the set. In each row, the dots represent the enriched biological terms. The size of each dot is proportional to the number of the transcripts enriched in the term, while the color of the dots is the p-adjusted value, spanning from red to blue. C Volcano plots of significantly upregulated transcripts (red) and significantly down-regulated transcripts (blue) based on p < 0.05 and fold change ≥ 1 or ≤−1, respectively; black dots represent non-differentially expressed transcripts. Transcripts with a P value equal to or lower than 10⁻¹⁰ were clustered together.