Fig. 2: Co-culture with HS5-CD40L-IL4 and HS5-CD40L-IL4-IL21 cells induces a gene profile similar to lymph node CLL cells. | Leukemia

Fig. 2: Co-culture with HS5-CD40L-IL4 and HS5-CD40L-IL4-IL21 cells induces a gene profile similar to lymph node CLL cells.

From: Stromal cells engineered to express T cell factors induce robust CLL cell proliferation in vitro and in PDX co-transplantations allowing the identification of RAF inhibitors as anti-proliferative drugs

Fig. 2

A Heatmap of differentially expressed mRNAs (Padj < 0.05) in CLL cells cultured (3 days, n = 4) on plastic or co-cultured on HS5-WT or HS5-CD40L-IL4. One sample cultured on plastic was excluded due to RNA/library preparation failure. For sample characteristics see Supplementary Table S1 (CLL No. 042, 054, 073, 067). B MYC, NFκB, E2F, and BCR gene expression signatures in CLL cells co-cultured (3 days) on HS5-WT or HS5-CD40L-IL4 cells (samples from panel [A], mRNAs with base mean ≥10). The signature genes were adopted from Herishanu et al. [3]. C Representative immunoblot of CLL cells co-cultured (40 h) with various engineered HS5 cell or their combinations (1:1 ratio). D Cell-surface IgM level in CLL cells co-cultured (48 h) on HS5-WT or HS5-CD40L-IL4 cells. E Heatmap of differentially expressed mRNAs (Padj < 0.05) in CLL cells co-cultured (n = 4, 3 days) on HS5-WT, HS5-CD40L-IL4, or HS5-CD40L-IL4-IL21 cells. One sample co-cultured on HS5-CD40L-IL4 was excluded due to RNA/library preparation failure. For sample characteristics see Supplementary Table S1 (CLL No. 089, 047, 043, 044; different samples from those in panel A). F Overlap of differentially expressed mRNAs (Padj < 0.05) in CXCR4dimCD5bright CLL subpopulation and in CLL cells co-cultured (3 days) on HS5-WT vs. HS5-CD40L-IL4 (i) or HS5-WT vs. HS5-CD40L-IL4-IL21 cells (ii). Gene expression of CXCR4dimCD5bright cells vs. co-cultured CLL cells had a significant overlap (P < 0.0001, Fisher exact test) for all 4 comparisons.

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