Fig. 5: Summary of the effects and utility of the CLL co-culture model with engineered HS5-CD40L-IL4 ± IL21 cells. | Leukemia

Fig. 5: Summary of the effects and utility of the CLL co-culture model with engineered HS5-CD40L-IL4 ± IL21 cells.

From: Stromal cells engineered to express T cell factors induce robust CLL cell proliferation in vitro and in PDX co-transplantations allowing the identification of RAF inhibitors as anti-proliferative drugs

Fig. 5

Co-culture with HS5 concurrently expressing CD40L and IL4 led to mild CLL cell proliferation (median 7% at day 7), while the HS5 concurrently expressing CD40L, IL4, and IL21 led to a high proliferation rate (median 44%). Pan-RAF inhibitors block CLL cell proliferation in co-cultures. The co-cultures mimicked the gene expression fingerprint of lymph node CLL cells (MYC, NFκB, and E2F signatures). Co-transplantation of HS5-CD40L-IL4±IL21 supportive cells on 3D collagen scaffold with primary CLL cells into immunodeficient NSG mice leads to B cell lymphoproliferative.

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