Fig. 2: Ddx41+/− erythroid progenitors have cell growth defect ex vivo and activated cell stress-related gene expression in vivo. | Leukemia

Fig. 2: Ddx41+/− erythroid progenitors have cell growth defect ex vivo and activated cell stress-related gene expression in vivo.

From: DDX41 haploinsufficiency causes inefficient hematopoiesis under stress and cooperates with p53 mutations to cause hematologic malignancy

Fig. 2

A Erythroid colony formation in methylcellulose by Lin− bone marrow cells from young mice of the indicated genotypes. B Ex vivo erythroid differentiation of Lin− bone marrow cells in liquid culture. The percentage of erythroid progenitors in each gate on the CD71/Ter119 flow cytometry assay is graphed. C Flow cytometry for erythroid progenitors by CD71/Ter119 in bone marrow of mice transplanted with cells of the indicated genotype. D Volcano plot for differential gene expression in RNA-Seq on R2 (CD71+ Ter119+) erythroid progenitors from the bone marrow of transplanted mice at 8wks post-transplant. E Heatmap of differentially expressed genes in the Cellular Response to Stress pathway from the Reactome database. F Enrichment of Reactome pathways in differentially expressed genes from RNA-Seq on R2 cells.

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