Fig. 3: Ddx41+/− hematopoietic stem and progenitor cells have modestly reduced fitness, correlating with reduced protein translation activity. | Leukemia

Fig. 3: Ddx41+/− hematopoietic stem and progenitor cells have modestly reduced fitness, correlating with reduced protein translation activity.

From: DDX41 haploinsufficiency causes inefficient hematopoiesis under stress and cooperates with p53 mutations to cause hematologic malignancy

Fig. 3

A Competitive transplant of bone marrow cells from Ddx41+/f;Rosa-CreERT2 and control mice against CD45.1+ competitor bone marrow cells. Peripheral blood chimerism was determined by flow cytometry for CD45.2 and CD45.1 for 16 weeks. B Chimerism in bone marrow cell populations determined by flow cytometry for CD45.2 and CD45.1. C Non-competitive transplant of bone marrow cells from Ddx41+/f;Rosa-CreERT2 and wild-type Rosa-CreERT2 mice was conducted, and Lin−Sca+Kit+ cells were harvested for colony formation assays in methylcellulose. D In vivo staining for protein translation rate by OP-Puro injection into transplanted mice in C followed by flow cytometry on bone marrow cells. Relative OP-puro signal in each cell population is graphed.

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