Fig. 5: Reduced DDX41 expression causes p53 activation and the effects of incomplete loss of DDX41 can be partially rescued by p53 loss. | Leukemia

Fig. 5: Reduced DDX41 expression causes p53 activation and the effects of incomplete loss of DDX41 can be partially rescued by p53 loss.

From: DDX41 haploinsufficiency causes inefficient hematopoiesis under stress and cooperates with p53 mutations to cause hematologic malignancy

Fig. 5

A The percentage of germline DDX41 MDS/AML patients with co-mutations in the indicated genes from five different patient cohort studies. B Western blot for DDX41 and p53 expression in MOLM13 cells expressing anti-DDX41 shRNAs. C The percentage of GFP+ cells in 1:1 mixed cultures of MOLM13 cells transduced with sh-control or sh-DDX41 and non-transduced MOLM13 cells. The vector for shRNA expression is pLKO.1-GFP. D Flow cytometry for AnnexinV and cell cycle in cultures of MOLM13 cells expressing anti-DDX41 shRNAs. E Measurement of protein translation activity by flow cytometry for HPG incorporation in MOLM13 cells expressing anti-DDX41 shRNAs compared to sh-control. F Measurement of DNA damage by flow cytometry for γH2AX in MOLM13 cells expressing anti-DDX41 shRNAs compared to sh-control. G The percentage of GFP+ cells in 1:1 mixed cultures of MOLM13 cells transduced with sh-control or sh-DDX41 cells and non-transduced cells. H Flow cytometry for AnnexinV in cultures of p53-knockout MOLM13 cells expressing anti-DDX41 shRNAs. I Colony formation from Lin− cells of the indicated genotypes in methylcellulose containing 4-OHT and cytokines permissive of myeloid or erythroid colony growth. J Peripheral blood chimerism measured by flow cytometry for CD45.2 in mice transplanted with a 1:1 mixture of cells of the indicated genotype and control CD45.1 cells.

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