Fig. 3: Prevalence and timing of mutational processes.

A Overview of clusters of mutations detected in case P0121 as a representative example. On the left are plots of the change in mutant allele frequency over time for all individual mutations in each cluster. Mutant allele frequency and the name of each cluster is displayed on the y-axis and the sample time points are on the x-axis. Mutations in a region with a copy number gain (blue) or loss (red) were colored. The middle plots represent the 96-trinucleotide mutational profiles corresponding to each cluster. On the right are barcharts displaying the absolute number of mutations (x-axis) attributed to color-coded mutational signatures in each cluster. Clusters with less than 200 mutations were not assigned mutational signatures and named unassigned. Furthermore, mutations attributed to mutational signatures which were assigned in less than 75% of bootstraps (see methods) were also classified as unassigned. B Fish plot of clonal development in case P0121. Colors correspond to the mutation clusters shown in A. Relative contribution of APOBEC-associated mutations to mutations acquired at each time point is depicted below the fish plot. C Relative contribution of SBS7a-associated mutations to acquired mutations per time point, in all four SBS7a-positive cases in the cohort. Color intensity represents the relative contribution. Broken lines indicate missing samples. D Relative contribution of mutations associated to SBS2 and SBS13 in six cases, depicted as in C.