Fig. 1: An atlas of preleukemic bone marrow stroma.
From: Cellular taxonomy of the preleukemic bone marrow niche of acute myeloid leukemia
A Schematic representation of experiment. Preleukemic PTD;ITD or age- and sex-matched WT mice were sacrificed. Femurs and tibias were collagenase digested and Lin- CD45- Ter119- CD71- stromal cells were sorted by flow cytometry. Sorted stromal cells were analyzed by 10x scRNA-seq. B t-Distributed stochastic neighbor embedding (t-SNE) of stromal cells, colored by clustering and annotated based on gene signature. C Dot plot of top expressed genes in stromal cells by clusters. D t-SNE of stromal cells by expression of key cell-type marker genes. E t-SNE of stromal cells colored by condition (WT, blue; preleukemic PTD;ITD, red). F Percentage of stromal cells across each cluster in WT and preleukemic PTD;ITD cells.
