Fig. 3: NPM1::MLF1 and NPM1::CCDC28A induce the development of transplantable AML in vivo.
From: NPM1-fusion proteins promote myeloid leukemogenesis through XPO1-dependent HOX activation
A–C Bone marrow cells were collected from the moribund mice bearing NPM1::MLF1 or NPM1::CCDC28A-expressing cells. BM bone marrow. A Representative flow cytometry profiles (right) and a bar graph showing the frequency of GFP+CD11b+ cells (left). Data are shown as means ± SEM. (NPM1::MLF1: n = 2, NPM1::CCDC28A: n = 6). B Representative flow cytometry profiles (right) and a bar graph showing the frequency of GFP+c-kit+ cells and GFP+ granulocytic-monocytic progenitors are shown. Data are shown as means ± SEM. Numbers indicate the frequency of each population. GMP granulocytic-monocytic progenitors. CMP common myeloid progenitors. MEP megakaryocyte/erythrocyte progenitors. C Wright-Giemsa staining of bone marrow cells collected from the recipient mice bearing NPM1::MLF1 or NPM1::CCDC28A-expressing cells. Scale bar, 20 μm. D Kaplan-Meier survival curve of secondary recipient mice transplanted with the NPM1::MLF1 or NPM1::CCDC28A-expressing cells (NPM1::MLF1, n = 6, NPM1::CCDC28A, n = 6).
